yxeI [2019-07-16 09:04:52]

penicillin acylase

locus: BSU_39540

pI: 5.28

mw: 37.04 kDa

protein length: 328 aa

gene length: 987 bp

function: resistance to penicillin

product: penicillin acylase

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]

Gene

Coordinates: 4,062,543 → 4,063,529

The protein

Catalyzed reaction/ biological activity

• acylation of penicillin V [Pubmed|21978958]

Protein family

• peptidase C59 family (according to Swiss-Prot)

Structure

• [PDB|2OQC]

Expression and Regulation

Operons

• genes: [gene|B5153BCBEFFEA973B99F8B54047EF6FD830CAEE8|yxeI]-[gene|2976D8A6F6F40BA3FF226CF7B67F25DD237373D1|yxeJ]
  description: [Pubmed|10746760]

Biological materials

Mutant

• MGNA-B708 (yxeI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1707 NBRP B. subtilis, Japan]
• BKE39540 (Δ[gene|B5153BCBEFFEA973B99F8B54047EF6FD830CAEE8|yxeI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGATCAGTCCCCTTTT, downstream forward: _UP4_ATTCATGAGCTTAATTAATT
• BKK39540 (Δ[gene|B5153BCBEFFEA973B99F8B54047EF6FD830CAEE8|yxeI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGATCAGTCCCCTTTT, downstream forward: _UP4_ATTCATGAGCTTAATTAATT

References

• 10746760,15937167,21978958